@prefix this: <https://w3id.org/np/RAayjZpU3XiN94dTuMD4RB4n-yFGgb83zHERb5LjLgBb0> .
@prefix sub: <https://w3id.org/np/RAayjZpU3XiN94dTuMD4RB4n-yFGgb83zHERb5LjLgBb0/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix pav: <http://purl.org/pav/> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .
@prefix rdfg: <http://www.w3.org/2004/03/trix/rdfg-1/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix npx: <http://purl.org/nanopub/x/> .

<https://w3id.org/np/RAayjZpU3XiN94dTuMD4RB4n-yFGgb83zHERb5LjLgBb0> a np:Nanopublication,
    npx:RoCrateNanopub;
  np:hasAssertion sub:assertion;
  np:hasProvenance sub:provenance;
  np:hasPublicationInfo sub:pubinfo;
  dct:created "2026-03-03T16:43:41.058+01:00"^^xsd:dateTime;
  dct:creator <https://w3id.org/kpxl/gen/terms/RoCrateBot>;
  npx:introduces <https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/>;
  rdfs:label "Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter" .

<http://eurovoc.europa.eu/4921> a <http://schema.org/DefinedTerm>;
  <http://schema.org/description> "";
  <http://schema.org/name> "Biology" .

<http://w3id.org/ro-id/rohub/model#subject/-107053379> a <https://w3id.org/contentdesc#Domain>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "biology" .

<http://w3id.org/ro-id/rohub/model#subject/-1217298392> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "reporter system" .

<http://w3id.org/ro-id/rohub/model#subject/-1651525790> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "Nat. Commun" .

<http://w3id.org/ro-id/rohub/model#subject/-2045192619> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "neuronal phenotype" .

<http://w3id.org/ro-id/rohub/model#subject/-574351702> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "fluorescent reporter" .

<http://w3id.org/ro-id/rohub/model#subject/-726690546> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "monogenic disease" .

<http://w3id.org/ro-id/rohub/model#subject/-900704710> a <https://w3id.org/contentdesc#Domain>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "medicine" .

<http://w3id.org/ro-id/rohub/model#subject/100003229> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "photon" .

<http://w3id.org/ro-id/rohub/model#subject/100007218> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "gene" .

<http://w3id.org/ro-id/rohub/model#subject/100007899> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "cell" .

<http://w3id.org/ro-id/rohub/model#subject/100012167> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "disease" .

<http://w3id.org/ro-id/rohub/model#subject/100041602> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "epilepsy" .

<http://w3id.org/ro-id/rohub/model#subject/100041834> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "gram" .

<http://w3id.org/ro-id/rohub/model#subject/100042940> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "reporter" .

<http://w3id.org/ro-id/rohub/model#subject/100043085> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "neuron" .

<http://w3id.org/ro-id/rohub/model#subject/100047304> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "central nervous system" .

<http://w3id.org/ro-id/rohub/model#subject/100104084> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "phenotype" .

<http://w3id.org/ro-id/rohub/model#subject/100755108> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "mosaicism" .

<http://w3id.org/ro-id/rohub/model#subject/101074984> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "microscopy" .

<http://w3id.org/ro-id/rohub/model#subject/127479> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "generation" .

<http://w3id.org/ro-id/rohub/model#subject/1293691923> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "mosaic model" .

<http://w3id.org/ro-id/rohub/model#subject/151609> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "mosaic" .

<http://w3id.org/ro-id/rohub/model#subject/1810438714> a <https://w3id.org/contentdesc#Domain>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "genetics" .

<http://w3id.org/ro-id/rohub/model#subject/187985994> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "GM. modelling" .

<http://w3id.org/ro-id/rohub/model#subject/2024019653> a <https://w3id.org/contentdesc#Organization>;
  <http://schema.org/name> "Commun" .

<http://w3id.org/ro-id/rohub/model#subject/23926> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "genotype" .

<http://w3id.org/ro-id/rohub/model#subject/458383126> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "knockout mosaic" .

<http://w3id.org/ro-id/rohub/model#subject/544869759> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "genetic mosaicism" .

<http://w3id.org/ro-id/rohub/model#subject/59620> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "inclusion" .

<http://w3id.org/ro-id/rohub/model#subject/60775> a <https://w3id.org/contentdesc#Concept>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "disorder" .

<http://w3id.org/ro-id/rohub/model#subject/610744183> a <https://w3id.org/contentdesc#Place>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "Trovato" .

<http://w3id.org/ro-id/rohub/model#subject/73186238> a <https://w3id.org/contentdesc#Place>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "Landi" .

<http://w3id.org/ro-id/rohub/model#subject/887141355> a <https://w3id.org/contentdesc#Expression>;
  <http://www.w3.org/2004/02/skos/core#prefLabel> "modelling genetic mosaicism" .

<https://orcid.org/0000-0002-2736-0052> a <http://xmlns.com/foaf/0.1/Agent>;
  <http://schema.org/email> "federica.foglini@ismar.cnr.it";
  <http://schema.org/name> "Federica Foglini" .

<https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/#enrichment_service-account-enrichment>
  a <http://xmlns.com/foaf/0.1/Agent>;
  <http://schema.org/name> "service-account-enrichment" .

<https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/> a <http://purl.org/wf4ever/ro#ResearchObject>,
    <http://purl.org/wf4ever/roevo#LiveRO>, <http://schema.org/Dataset>, <http://w3id.org/ro/earth-science#BibliographyResearchObject>,
    <https://w3id.org/ro/terms/earth-science#BibliographyResearchObject>;
  <http://schema.org/about> <http://eurovoc.europa.eu/4921>;
  <http://schema.org/author> <https://orcid.org/0000-0002-2736-0052>;
  <http://schema.org/contentSize> 5271;
  <http://schema.org/contentUrl> "https://api.rohub.org/api/ros/9641a891-0c97-4a21-bcc5-3c0e7f974ede/crate/download/";
  <http://schema.org/creator> <mailto:service-account-generation-service>;
  <http://schema.org/dateCreated> "2021-12-10 10:05:58.607206+00:00";
  <http://schema.org/dateModified> "2025-03-05 00:56:54.503963+00:00";
  <http://schema.org/datePublished> "2021-12-10 10:05:58.607206+00:00";
  <http://schema.org/description> "Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present in monogenic diseases of the central nervous system caused by the random inactivation of the X-chromosome, in the case of X-linked pathologies, or by somatic mutations affecting a subset of neurons. The comprehension of the mechanisms of these diseases and of the cell-autonomous effects of specific mutations requires the generation of sparse mosaic models, in which the genotype of each neuron is univocally identified by the expression of a fluorescent protein in vivo. Here, we show a dual-color reporter system that, when expressed in a floxed mouse line for a target gene, leads to the creation of mosaics with tunable degree. We demonstrate the generation of a knockout mosaic of the autism/epilepsy related gene PTEN in which the genotype of each neuron is reliably identified, and the neuronal phenotype is accurately characterized by two-photon microscopy.";
  <http://schema.org/encodingFormat> "application/ld+json";
  <http://schema.org/hasPart> <https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/resources/863f7285-2401-467b-9d3b-485498dc267d>;
  <http://schema.org/identifier> "https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede";
  <http://schema.org/license> <https://choosealicense.com/no-permission/>;
  <http://schema.org/name> "Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter";
  <http://w3id.org/ro-id/rohub/model#creation_mode> "MANUAL";
  <https://www.w3.org/ns/iana/link-relations/relation#cite-as> "Foglini, Federica. \"Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter.\" ROHub. Dec 10 ,2021. https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede." .

<https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/resources/863f7285-2401-467b-9d3b-485498dc267d>
  a <http://purl.org/wf4ever/wf4ever#Resource>, <http://schema.org/MediaObject>;
  <http://schema.org/author> <mailto:service-account-generation-service>;
  <http://schema.org/contentSize> 346;
  <http://schema.org/contentUrl> "https://api.rohub.org/api/resources/863f7285-2401-467b-9d3b-485498dc267d/download/";
  <http://schema.org/creator> <mailto:service-account-generation-service>;
  <http://schema.org/dateCreated> "2021-12-10 10:06:01.685172+00:00";
  <http://schema.org/dateModified> "2021-12-10 10:06:01.686020+00:00";
  <http://schema.org/description> "Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present in monogenic diseases of the central nervous system caused by the random inactivation of the X-chromosome, in the case of X-linked pathologies, or by somatic mutations affecting a subset of neurons. The comprehension of the mechanisms of these diseases and of the cell-autonomous effects of specific mutations requires the generation of sparse mosaic models, in which the genotype of each neuron is univocally identified by the expression of a fluorescent protein in vivo. Here, we show a dual-color reporter system that, when expressed in a floxed mouse line for a target gene, leads to the creation of mosaics with tunable degree. We demonstrate the generation of a knockout mosaic of the autism/epilepsy related gene PTEN in which the genotype of each neuron is reliably identified, and the neuronal phenotype is accurately characterized by two-photon microscopy.";
  <http://schema.org/encodingFormat> "text/plain";
  <http://schema.org/license> <https://choosealicense.com/no-permission/>;
  <http://schema.org/name> "Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter";
  <http://schema.org/sdDatePublished> "2021-12-10 10:06:01.685172+00:00" .

<https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/ro-crate-metadata.json>
  a <http://schema.org/CreativeWork>;
  dct:conformsTo <https://w3id.org/ro/crate/1.1>;
  <http://schema.org/about> <https://w3id.org/ro-id/9641a891-0c97-4a21-bcc5-3c0e7f974ede/> .

<mailto:service-account-generation-service> a <http://xmlns.com/foaf/0.1/Agent>;
  <http://schema.org/name> "service-account-generation-service" .

sub:assertion prov:wasDerivedFrom <https://api.rohub.org/api/ros/9641a891-0c97-4a21-bcc5-3c0e7f974ede/crate/download/ro-crate-metadata.json> .

sub:sig npx:hasAlgorithm "RSA";
  npx:hasPublicKey "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAxszSDYX5tuCSkP7UiCtftYPFNQVTjgNu0I5fwdML2DLRDlp0xzmsQXRk8oHuvwGvG1aMjj6cpUqO+0rz2Sg/wvHOgUpkRH8VJXvmlkhafMLCMtUtk5JIx7e+fkzCby+fnmD7kMkGLrT+OaExWwEDmNlCAt0TPKcHSdwsjso2isXjtAsGevyCMke8ufnFYpjs746JES1eNzVnHnn2Kp/lqcm60GM+J8dLgRZp7fX0anW098xhKym6+xXFzqeju0vYRIHBPerv+r7skWxwk+a7Sd8msqVeYEv6NTqnyWvyWb6Yh8cvj04N6qm/T6C5FUPLQhzSaQgMVMU6yLqjPuu9DwIDAQAB";
  npx:hasSignature "KjzSOf8ujnmksonL5S0apEj0oMq9oE/H7eFJ9bU3JWxCeorAV2IPmN73fxf0YACM3tzfk+/QNevgVnlPBjDSO6XBh6aDcAWYh9PH6BNhyVqDONo5G962AyJeiiaRFvzzjJ6pq2uW1vANrmTJ0O1zFUy4rSG8xXaSLEaCTAhXurtnNaenN3Xzme4of5Yt8ZS2VaMv3CW2V2euISwUqrelIzDeJpgPkzChJk5PnphY/zUV/GURAswL42LEa6KfYfGvpn7Wk1HeiUm9p29Dj3ZAa7V9mOTM/iMXLx0iV4MKwTLh9+MlZnHSJ5tSsBSRb6FE90ul0ncstRG4lG9AjIggsg==";
  npx:hasSignatureTarget <https://w3id.org/np/RAayjZpU3XiN94dTuMD4RB4n-yFGgb83zHERb5LjLgBb0>;
  npx:signedBy <https://w3id.org/kpxl/gen/terms/RoCrateBot> .