RAL4fGVNEl (explore)

Nanopublication: RAL4fGVNEl

Full identifier: http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g

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RAL4fGVNEl Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_10 _10 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1% SDS" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1% Triton -X" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_4 _4 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "micropistle" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_5 _5 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1.5 ml eppendorf tube" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_6 _6 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1X STE buffer" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_7 _7 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Proteinase K (20mg/ml)" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_8 _8 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "100mM sucrose" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_9 _9 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Rnase A (20mg/ml)" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step http://purl.org/dc/elements/1.1/description description (this is a literal) "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_1 _1 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step http://www.w3.org/1999/02/22-rdf-syntax-ns#type type http://purl.org/net/p-plan#Step Step .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_3 _3 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_4 _4 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_5 _5 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_10 _10 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_2 _2 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_6 _6 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_7 _7 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_8 _8 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_9 _9 .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion The assertion above http://purl.org/pav/createdWith createdWith https://nanotate.bitsfetch.com nanotate.bitsfetch.com .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime generatedAtTime (this is a literal) "2021-03-10T10:33:19.355370" .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo wasAttributedTo https://hypothes.is/users/alejandra.sandoval alejandra.sandoval .

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#step step .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#sig sig http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal) "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#sig sig http://purl.org/nanopub/x/hasSignature has the signature (this is a literal) "Kd4Aa6fDdpMWhH77AgLNFo/GE7w50Y0bXMfwIOIVdP+2Jp7DcBZ3K28i9HOzqkwNBC9YZmAKwLlwHp818FZw1VIUbdMpPIaCSwdAsSJgsv/Ov/vuO6093NjHOignVj4QsE9+XO9WZOyFBX1XxDAbO2anaVMuoEQa4HYFefmlDOc=" .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#assertion the assertion above http://www.w3.org/ns/prov#wasDerivedFrom wasDerivedFrom https://hypothes.is/a/8b4bAHJNEeu01RN1eV0q-Q 8b4bAHJNEeu01RN1eV0q-Q .

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g this nanopublication http://www.w3.org/ns/prov#generatedAtTime generatedAtTime (this is a literal) "2021-03-10T10:33:19.355370" .

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g this nanopublication http://www.w3.org/ns/prov#wasAttributedTo wasAttributedTo https://hypothes.is/users/alejandra.sandoval alejandra.sandoval .

Nanotate, 10 Mar 2021, 10:33:19 UTC

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