step (references)

References for: step

Full identifier: http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#step

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
http://purl.org/np/RAQ8Vn8NkpbiTE_KaX-qBoC5xbCrkmBBHwMAj8aoBH1ck RAQ8Vn8Nkp	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	http://purl.org/np/RA0vn8x1MSb1EnQG63epRIlQXDdmTuxnRHz4tskxoUqL4#step step	Nanotate	2021-04-21T14:44:11.546Z
http://purl.org/np/RAQ8Vn8NkpbiTE_KaX-qBoC5xbCrkmBBHwMAj8aoBH1ck RAQ8Vn8Nkp	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan	http://purl.org/np/RAQ8Vn8NkpbiTE_KaX-qBoC5xbCrkmBBHwMAj8aoBH1ck#plan plan	Nanotate	2021-04-21T14:44:11.546Z
http://purl.org/np/RAQ8Vn8NkpbiTE_KaX-qBoC5xbCrkmBBHwMAj8aoBH1ck RAQ8Vn8Nkp	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	http://purl.org/np/RAJODcXkxbQqP3lyEUrcmEwNwlUO6Wq4HwMxF62hjjF74#step step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	step	Nanotate	2021-04-21T14:44:11.546Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed bioSampleUsed	http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#_1 _1	Nanotate	2021-03-10T11:18:25.462Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed reagentUsed	http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#_2 _2	Nanotate	2021-03-10T11:18:25.462Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.w3.org/1999/02/22-rdf-syntax-ns#type type	http://purl.org/net/p-plan#Step Step	Nanotate	2021-03-10T11:18:25.462Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/dc/elements/1.1/description description	Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.	Nanotate	2021-03-10T11:18:25.462Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed labEquipmentUsed	http://purl.obolibrary.org/obo/OBI_0400155 OBI_0400155	Nanotate	2021-03-10T11:18:25.462Z
http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo	http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo RAMb6wb2si	http://purl.org/nanopub/x/introduces introduces	step	Nanotate	2021-03-10T11:18:25.462Z