References for: step

Full identifier: http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step

Nanopublication Part Subject Predicate Object Published By Published On
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:32:30.020Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:32:30.020Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion
step
Nanotate
2021-02-25T16:19:32.955Z
links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo
step
Nanotate
2021-02-25T16:19:32.955Z