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step

Full identifier: http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step

Assigned to 1 class:

http://purl.org/net/p-plan#Step Step ( )

Described in 1 nanopublication:

http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T ( )

Minted in Nanopublication

RA6U6VQH2T Step comment approve/disapprove edit as derived nanopublication

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Nanotate, 14 Feb 2021, 05:25:31 UTC

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
http://purl.org/np/RAtGrYaeHV... RAtGrYaeHV	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan	http://purl.org/np/RAtGrYaeHV...#plan plan	Nanotate	2021-03-05T14:32:20.677Z
http://purl.org/np/RAtGrYaeHV... RAtGrYaeHV	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	http://purl.org/np/RAPv8Hp5eg...#step step	Nanotate	2021-03-05T14:32:20.677Z
http://purl.org/np/RAtGrYaeHV... RAtGrYaeHV	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	http://purl.org/np/RAEfoaUX0G...#step step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	step	Nanotate	2021-03-05T14:32:20.677Z
http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.w3.org/1999/02/22-rdf-syntax-ns#type type	http://purl.org/net/p-plan#Step Step	Nanotate	2021-02-14T05:25:31.936Z
http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/dc/elements/1.1/description description	Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates.	Nanotate	2021-02-14T05:25:31.936Z
http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed	http://purl.org/np/RA6U6VQH2T...#_1 _1	Nanotate	2021-02-14T05:25:31.936Z
http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed	http://purl.org/np/RA6U6VQH2T...#_2 _2	Nanotate	2021-02-14T05:25:31.936Z
http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo	http://purl.org/np/RA6U6VQH2T... RA6U6VQH2T	http://purl.org/nanopub/x/introduces introduces	step	Nanotate	2021-02-14T05:25:31.936Z

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