_1 (explore) | nanodash

_1

http://purl.org/np/RA8LXJxDVioLwvnT7U0GREq_szXgcjzp3WYAyInmPlWNQ#_1

Minted in Nanopublication

RA8LXJxDVi Step comment approve/disapprove edit as derived nanopublication

http://purl.obolibrary.org/obo/OBI_0400155

OBI_0400155

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"water bath"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#_1

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"lysozyme solution"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

http://purl.org/dc/elements/1.1/description

description

(this is a literal)

"Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield."

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

http://www.w3.org/1999/02/22-rdf-syntax-ns#type

type

http://purl.org/net/p-plan#Step

Step

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

http://www.bioassayontology.org/bao#BAO_0002104

BAO_0002104

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

http://purl.obolibrary.org/obo/OBI_0400155

OBI_0400155

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#_1

http://www.bioassayontology.org/bao#BAO_0002104

BAO_0002104

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"suspension"

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion The assertion above http://purl.org/pav/createdWith

createdWith

https://nanotate.bitsfetch.com

nanotate.bitsfetch.com

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-02-26T09:44:15.524571"

(xsd:dateTime) .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/zuluaga.lizzz

zuluaga.lizzz

This is the identifier for this whole nanopublication. http://purl.org/np/RA8LXJxDVi... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#step

step

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#sig

sig

http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RA8LXJxDVi... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#sig

sig

http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal)

"RSA"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#sig

sig

http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal)

"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA8LXJxDVi...#sig

sig

http://purl.org/nanopub/x/hasSignature has the signature (this is a literal)

"fHtQyp6CR1dzknNYSulii53Kpdx63g3ELHhARErrwVgcSxWDhvAeQhEPO6hAGppZitcTqNsyFZd9SXgrBy11gVFRB7ghF97zELzr5YgycWfkPd+ggsnjOS/EvFa/Dyx3R6eRdNOV36npp0akvuhqvFSDIer7hkd5VxmNTm08B7k="

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/Mm4SaHeyEeupA9_TrHGoQQ

Mm4SaHeyEeupA9_TrHGoQQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/SxYACneyEeuhZweY7act7g

SxYACneyEeuhZweY7act7g

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/f0Q7MHeyEeum_5cx2E1wjg

f0Q7MHeyEeum_5cx2E1wjg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA8LXJxDVi...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/osRxYHeyEeu5zVuedDPw0Q

osRxYHeyEeu5zVuedDPw0Q

This is the identifier for this whole nanopublication. http://purl.org/np/RA8LXJxDVi...

this nanopublication

http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-02-26T09:44:15.524571"

(xsd:dateTime) .

This is the identifier for this whole nanopublication. http://purl.org/np/RA8LXJxDVi...

this nanopublication

http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/zuluaga.lizzz

zuluaga.lizzz

Nanotate, 26 Feb 2021, 09:44:15 UTC

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
http://purl.org/np/RA8LXJxDVi... RA8LXJxDVi	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	_1	http://www.w3.org/2000/01/rdf-schema#label label	lysozyme solution	Nanotate	2021-02-26T09:44:15.524Z
http://purl.org/np/RA8LXJxDVi... RA8LXJxDVi	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	http://purl.org/np/RA8LXJxDVi...#step step	https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed	_1	Nanotate	2021-02-26T09:44:15.524Z

Raw

TriG(txt), JSON-LD(txt), N-Quads(txt), XML(txt)