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step

Full identifier: http://purl.org/np/RAYDwTtT3SWmPi5y6QPlgud6dumB_mJt6XQ3oYluNNiVU#step

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Minted in Nanopublication

RAYDwTtT3S Step comment approve/disapprove edit as derived nanopublication

http://purl.obolibrary.org/obo/CHEBI_17992 CHEBI_17992 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "100mM sucrose" .

http://purl.obolibrary.org/obo/CHEBI_29234 CHEBI_29234 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1% Triton -X" .

http://purl.obolibrary.org/obo/CHEBI_8984 CHEBI_8984 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1% SDS" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_1 _1 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_2 _2 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "mosquitoes" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_3 _3 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "micropistle" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_4 _4 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Rnase A" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_5 _5 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "Proteinase K" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_6 _6 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1X STE buffer " .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step http://purl.org/dc/elements/1.1/description description (this is a literal) "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_1 _1 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step http://www.w3.org/1999/02/22-rdf-syntax-ns#type type http://purl.org/net/p-plan#Step Step .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_2 _2 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_3 _3 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed http://www.bioassayontology.org/bao#BAO_0010014 BAO_0010014 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed http://purl.obolibrary.org/obo/CHEBI_17992 CHEBI_17992 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_4 _4 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_5 _5 .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#_6 _6 .

http://www.bioassayontology.org/bao#BAO_0010014 BAO_0010014 http://www.w3.org/2000/01/rdf-schema#label label (this is a literal) "1.5 ml eppendorf tube" .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAYDwTtT3S...#assertion The assertion above http://purl.org/pav/createdWith createdWith https://nanotate.bitsfetch.com nanotate.bitsfetch.com .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAYDwTtT3S...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime generatedAtTime (this is a literal) "2021-02-24T15:57:29.331725" .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAYDwTtT3S...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo wasAttributedTo https://hypothes.is/users/zuluaga.lizzz zuluaga.lizzz .

This is the identifier for this whole nanopublication. http://purl.org/np/RAYDwTtT3S... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#step step .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#sig sig http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAYDwTtT3S... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#sig sig http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal) "RSA" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#sig sig http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal) "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAYDwTtT3S...#sig sig http://purl.org/nanopub/x/hasSignature has the signature (this is a literal) "aWhdnmGhJ66HdQT7ErQofYYVZALQSx0vSYV2bxZ9SyR8RnJ6zgt68Y6GIWNMMc6hLBRV5YxFXMEs4JA8KehE8EK+QlYjhEiP1q5Km6xmWcFngNWmrVfg2HQbw7jgUlZ6licoQwCN1GmXIv57ElrxNKIP3ghmVtOCEqodFcpRE8E=" .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAYDwTtT3S...#assertion the assertion above http://www.w3.org/ns/prov#wasDerivedFrom wasDerivedFrom https://hypothes.is/a/1x5l6HbfEeuNLNOMl31uWw 1x5l6HbfEeuNLNOMl31uWw .

This is the identifier for this whole nanopublication. http://purl.org/np/RAYDwTtT3S... this nanopublication http://www.w3.org/ns/prov#generatedAtTime generatedAtTime (this is a literal) "2021-02-24T15:57:29.331725" .

This is the identifier for this whole nanopublication. http://purl.org/np/RAYDwTtT3S... this nanopublication http://www.w3.org/ns/prov#wasAttributedTo wasAttributedTo https://hypothes.is/users/zuluaga.lizzz zuluaga.lizzz .

Nanotate, 24 Feb 2021, 15:57:29 UTC

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